Hybridization

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  • Mixing a set of atomic orbitals to form a new set of atomic orbitals with the same total electron capacity and with properties and energies intermediate between those of the original unhybridized orbitals.
  • The hydrogen bonding of complementary DNA and/or RNA sequences to form a duplex molecule.
  • Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing. In a reversal of this process, a double-stranded DNA (or RNA, or DNA/RNA) molecule can be heated to break the base pairing and separate the two strands. Hybridization is a part of many important laboratory techniques such as polymerase chain reaction and Southern blotting.


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